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2006-2007 RESEARCH PLAN
TABLE OF CONTENTS

SCCWRP 2006/07 Research Plan

WATERSHEDS
A1    Assessment of Water Quality and Loadings From Natural Landscapes
A2    Development and Evaluation of Watershed Models
A3     Development of Linked Watershed-Estuarine Hydrodynamic and Water Quality Models
A4     Effects of Regionwide Fires on Deposition, Runoff, and Emissions to the Southern California Bight
A5     Refinement of Freshwater Bioassessments in Southern California
A6     Development of Bioindicators for Ephemeral Streams
A7     Bioaccumulation in Fishes Consumed by Anglers in Ventura and Los Angeles County Watersheds
A8     Characteristics of Effluents From Large Municipal Wastewater Treatment Facilities
A9     Large and Small Scale Deposition of Atmospheric Trace Metals in Southern California
A10    Comparison of Mass Emissions Among Sources in the Southern California Bight

WETLANDS AND ESTUARIES
B1     Historic Ecology of Southern California's Coastal Watershed and Wetlands
B2     Technical Support for Development of Nutrient Criteria for Coastal Estuaries and Lagoons
B3     Relationships Between Dissolved Oxygen and Algae Distribution in Newport Bay
B4     Source Apportionment of Pesticides in the Upper Newport Bay Watershed
B5     Investigations of Contaminants in the Upper Newport Bay Piscivorous Food Web
B6     Effectiveness of Treatment Wetlands as Stormwater BMPs and Compatibility with Wildlife Beneficial Uses
B7     Regional Monitoring/Assessment Program for Southern California Wetlands
B8     Landscape-scale Assessment of Southern California Riparian Ecosystems Condition
B9     Southern California Wetland Recovery Project Science Advisory Panel

COASTAL OCEAN
C1     Development of Sediment Quality Objectives for Bays and Estuaries
C2     Development of Methods to Characterize Sediment Toxicity in the Southern California Bight
C3     In-situ Measurements of Toxic Organic Compounds in Sediment Porewater
C4     Estimating Pollutant Loadings and Fluxes in Impaired Coastal Waterways
C5     Emerging Contaminants of Concern in Coastal Waters, Sediments, and Biota
C6     Endocrine Disruption in Coastal Fish
C7     Evaluation of the impact of Terrestrial Nutrient Runoff on the Biological Responses of the Coastal Ocean
C8     Relative Contaminant Concentrations in Whole Fish, Liver, and muscle Tissue in Demersal Fishes Used in Environmental Monitoring

BEACHES AND SHORELINES
D1     Epidemiology Study to Assess Swimmer Health Risk from NPS Sources of Bacteria
D2     Rapid Indicator Methodology for Measuring Fecal Indicator Bacteria
D3     Storm Drains and Sediments as Reservoirs of Fecal Indicator Bacteria
D4     Statewide Microbiology Monitoring Database
D5     MARINe: Multi-Agency Rocky Intertidal Network

REGIONAL MONITORING AND ASSESSMENT
E1     Southern California Bight Regional Monitoring: 2003
E2     Western Environmental Monitoring and Assessment Program (EMAP)
E3     Augmenting Fixed Grid Designs to Improve Local Mapping of Environmental Conditions in the Southern California Bight
E4     Statewide Assessment of Wetlands Status and Trends
E5     Statewide Surface Water Ambient Monitoring Program Data Management Node
E6     Web Based Data Discovery and Analysis Tool
E7     Southern California Coastal Ocean Observing System

COOPERATIVE RESEARCH
F1     Member Agency Technical Consulting

SCCWRP 2006/07 Research Plan

Welcome to the SCCWRP 2006/07 Research Plan. This Plan describes 38 projects, laid out by habitat, which demonstrates the range of interdisciplinary science we conduct and illustrates both the process-oriented and applied types of studies we perform. Although there are four different habitats targeted in this Plan (watersheds, wetlands and estuaries, beaches and shorelines, and the coastal ocean), you will see a thread of five common research themes throughout each habitat.

The first research theme is understanding background contaminant concentrations and natural variability (Water quality and loadings from natural landscapes, Historic ecology of southern California wetlands, Sediments as reservoirs of fecal indicator bacteria). This research helps put into focus what our environmental conditions should (or could) be and provides a baseline for comparison to areas where anthropogenic inputs are known to occur.

The second research theme is identifying and quantifying sources of anthropogenic pollutants. Some of these sources we have been tracking for decades (Characteristics of effluents from municipal wastewater facilities), while other projects explore new sources and types of contaminants (Large and small scale atmospheric deposition, Emerging contaminants of concern). Sometimes, our ideas require the development of new technology to be effective (Source apportionment of pesticides in Newport Bay, In situ measurements of toxic organic compounds in sediment porewater).

The third research theme is development of assessment tools. Some of these tools are for assessing impacts to human health (Epidemiology study of beaches impacted by nonhuman sources of fecal indicator bacteria, Bioaccumulation in fishes consumed by freshwater anglers) and others are for biological systems (Refinement of freshwater bioassessments, Development of bioindicators for ephemeral streams). Some projects are specifically directed towards setting thresholds for protecting ecosystems (Development of sediment quality objectives for bays and estuaries, Technical support for development of nutrient criteria). Altogether, the goal of this research theme is to enable managers to determine if environmental resources are at risk from manmade impacts.

The fourth research theme is understanding how management actions can affect positive changes by mitigating potential impacts (Development of watershed models, Effectiveness of treatment wetlands as stormwater BMPs, Evaluation of the impact of terrestrial runoff on biological responses in the coastal ocean). This research helps managers determine not only the most effective methods for reducing anthropogenic impacts, but also with the greatest cost-efficiency.

Our fifth research theme comprises projects dedicated towards bringing scientists and stakeholders together to achieve common regional, statewide, and national goals (Southern California Bight regional monitoring, Southern California Ocean Observing System, Statewide assessment of wetland status and trends, Western Environmental Monitoring and Assessment Program). These programs cover a wide array of disciplines including microbiology, oceanography, remote sensing, chemistry, toxicology, and biology. One area in particular that requires regional coordination is data management and SCCWRP is focused on coalescing and distributing not just data, but information (Web based data discovery and analysis tools, Augmenting fixed grid designs to improve local mapping, Statewide microbiology database).

As you can see, this years’ Research Plan pursues research themes that address some of the most pressing needs of the southern California environmental management community. By spreading these research themes across the habitats of greatest concern, SCCWRP can place the most salient information into the hands of both the regulated and regulatory agencies that use our products to improve their decision-making and stewardship of our natural resources.

WATERSHEDS

A1     ASSESSMENT OF WATER QUALITY AND LOADINGS FROM NATURAL LANDSCAPES
           Lead Investigator: E. Stein

Objectives: One of the challenges in evaluating watershed loading of potential pollutants is quantifying the relative fractions attributable to anthropogenic sources from urban areas versus natural contributions from undeveloped areas. Natural contributions of trace metals, nutrients, and bacteria can arise from the underlying geology, land cover and wildlife. For example, the Monterey shale formation that is common in southern California watersheds has been reported to be a source of phosphate loadings, which may contribute to algal growth in streams or estuaries. Trace metals, which are a cause of impairment in many watersheds, occur naturally in the environment and may leach as the underlying geology weathers. Land cover/vegetation type can also contribute to nutrient loadings in a watershed. For example, grasslands (both native and non-native) and the underlying soils have been shown to contribute relatively high loadings of nitrogen following rainfall events. These loadings contribute to the total nitrate and nitrite concentrations and may play a role in algae levels in streams and estuaries.

Data on contributions of potential pollutants from undeveloped lands during both wet and dry weather are lacking. Managers are often forced to use data from other parts of the country or anecdotal data from previous time periods as an estimate of natural contributions. This is especially evident in situations where a total maximum daily load (TMDL) is required. Without information on natural contributions, TMDLs may be developed with inefficient waste load allocations required to meet numeric targets.

The goal of this project is characterize the flow, algae, suspended solids, organic carbon, nutrients, metals, and bacteria from natural landscapes, and relate these to watershed properties such as geology, soil type, and vegetative cover. The objective is to evaluate water quality contributions and properties of stream reaches in undeveloped catchments throughout southern California’s coastal watersheds. Ultimately, this project will provide a characterization of natural baseline loadings associated with specific geologic settings and natural land cover/habitat types that will assist environmental managers with load allocations and setting appropriate numeric targets.

Tasks: Compilation of Existing Data Sources and Stakeholder Coordination. Reference watershed studies such as this have been few and relatively isolated. This task will identify, obtain, and summarize existing water quality data collected to date. These data will be used to help refine the sampling design and identify potential sampling locations for subsequent tasks. We will coordinate these decisions through a consensus of vested stakeholders.

Watershed Characterization and Site Selection. Major watersheds in southern California will be characterized in terms of their dominant physical and biological characteristics, such as geology, land cover, size, and slope. These characteristics will be defined using GIS, remote sensing, historical data (from the previous task), and local knowledge. These data will be used to select a series of sites that represent the range of factors that are assumed to affect variability in loadings from natural systems.

Dry and Wet Weather Sampling. Each site will be sampled during both storm (wet) and non-storm (dry) conditions. Data will be collected on flow, total dissolved and suspended solids, hardness, dissolved organic carbon, nutrients, trace metals, bacteria, and algae from each site. Sampling will occur over a two-year period, and data will be compiled into a database for analysis and dissemination. For bacteria, samples will be collected weekly throughout the year and daily for the three days following storms. For other constituents, samples will be collected quarterly and during 2-4 storm events.

Development of Assessment Tools and Evaluation of Management Measures. This task will assimilate the results of the field data and create tools that can be used in the development and implementation of TMDLs, watershed plans, and other management measures. We will develop profiles of loading from reference catchments based on their geologic setting and dominant land cover. These profiles will provide information on reference conditions for use in regional wetlands monitoring and assessment programs currently being developed in southern California. In addition, these profiles may be used to develop elements of a water quality model for undeveloped portions of local watersheds. These models can be used to investigate the effect of a change in loading from the undeveloped portions of the modeled watershed on downstream water quality.

Project Status: This is the third year of a three-year project. The first year focused on compilation and review of existing data sources and site selection. During the second year, 17 wet-weather site events and 44 dry weather site events were collected at 22 sampling sites in 10 different watersheds. The third year will focus on continued sampling during both dry and wet weather and on data analysis.

Collaborators: This project is being conducted in collaboration with University of California at Los Angeles and is partially funded by the Los Angeles Regional Water Quality Control Board (RWQCB) and the United States (US) Environmental Protection Agency (EPA) Region IX.



A2     Development and Evaluation of Watershed Models
          Lead Investigator: E. Stein

Objectives: Watershed models are important tools for effective watershed management. The value of watershed models is three-fold. First, model development requires a thorough understanding of the sources of pollution. This enables managers to assess where and when pollutants are entering their system. Second, model development requires an understanding of pollutant transport and transformation processes within the watershed. Knowing the transport and transformation of pollutants enables managers to link sources of pollution with impacts downstream, assess the potential for cumulative impacts, and identify the important locations or time periods that have the greatest influence on the mechanisms and processes that control their system. Third, and perhaps most important, watershed models can be predictive. Before managers implement BMPs to improve water quality, a predictive model allows them to evaluate the likely effectiveness of various management scenarios. By comparing the predicted improvement in water quality for numerous management strategies, the most efficient option can be identified and refined to achieve the greatest water quality enhancement for the least cost.

There are only a limited number of watersheds that have been modeled in the SCB and rarely have these modeling efforts been rigorously validated. For example, models such as hydrologic simulation model-Fortran (HSPF) have been used extensively in other parts of the country, but were developed primarily for open and agricultural watersheds and refined in watersheds where rainfall is frequent and rivers and creeks have perennial flow. It is unclear whether models developed for other regions of the country, such as HSPF, can perform adequately in the arid, urban watersheds of the SCB. SCB storms are typically very brief, but intense and no one has rigorously evaluated how well this model, or any other dynamic model, performs under these conditions. Factors such as variations in rainfall intensity and duration, antecedent dry period, as well as characteristics of various land use have never been thoroughly examined as elements of modeling error. For any model to be useful to regulators and stakeholders in the SCB, it must provide an accurate characterization of hydrologic process and pollutant washoff dynamics over the entire range of rainfall and pollutant build-up conditions.

The goal of this project is to develop, validate, and evaluate watershed fate and transport models for several watersheds in Southern California including the Ballona Creek, Los Angeles River, Dominguez Channel, Chollas Creek, Paleta Creek and San Gabriel River watersheds. We intend to use HSPF and evaluate its ability to accurately simulate a variety of existing conditions in urban, arid watersheds. We will then facilitate technology transfer to local stakeholders who may wish to use these models. Several stakeholders have already acknowledged their desire to apply these models in the development of TMDLs and associated implementation strategies.

Tasks: Assessment of data needs. The first task will be to assess what data is available and what data still needs to be collected to support model development. We will accomplish this task by conducting a retrospective analysis of historical monitoring data. These results can also be used for the TMDL problem statement in the study watersheds.

Dry weather surveys. Dry weather surveys are aimed towards identifying and quantifying sources of flow and pollutants to the river system of the study watershed. These surveys are designed to be large-scale snapshots of inputs that include point sources (i.e., water reclamation plants or industrial facilities) and nonpoint sources (i.e., storm drains or groundwater). These surveys are conducted by coordinating dozens of sampling teams that fan out over an entire watershed and collect samples from all flowing inputs over a short (ca. 4 hr) time scales.

Special Studies. Once the inputs to a river system are quantified, modelers need to understand how these inputs are transported and transformed as they move downstream in order to predict in-river concentrations. Many of the factors that control these variables are important rate controlling steps in watershed function. Therefore, we will conduct special studies to develop this information in order to calibrate and validate these key model parameters. Potential special studies include dye studies to quantify velocity, transport, dispersion and/or dilution, nitrification/denitrification rates, algal uptake and biomass, or temporal variability in storm drain concentrations.

Wet weather sampling. Unlike dry weather, where only a subset of storm drains flow and they can be sampled directly, wet weather input of flow and water quality need to be estimated using a land use runoff model. This type of approach assumes that the fraction of runoff from a particular land use type, such as residential, commercial, industrial, or open land use types, have a characteristic runoff signature in terms of imperviousness and water quality characteristics. For a given storm, the amount of runoff volume and pollutant loading can therefore be predicted. However, quantifying this signature is difficult because runoff coefficients and pollutant build up and washoff can vary greatly over a variety of storm conditions such as precipitation volume, intensity, and duration as well as antecedent moisture conditions. Therefore, we will collect rainfall, flow and water quality data at each of the modeled land use types over a variety of rainfall and antecedent moisture conditions. Multiple samples (n > 10) will be collected over the course of the targeted storm events and analyzed separately to understand within storm variability. Ultimately, these plots of concentration over time will help the modelers predict pollutant build-up and wash-off phenomenon that will be important in assessing BMP effectiveness for treating a storm’s first flush.

Development of watershed models for flow, nutrients, bacteria and trace metals. Wet and dry weather data will be used to develop model application for flow, nutrients, and trace metals in southern California coastal watersheds. First we will calibrate the model by recreating the time concentration series of pollutants at each of the modeled land uses. Then, after summing all of the land uses within the study watershed, we will validate the model by comparing the predicted time variable flow and pollutant time concentration series to sampled storm events at a downstream point in the watershed.

Model evaluation and sensitivity analysis. Existing watershed models will be expanded to investigate their sensitivity to a variety of input parameters at different temporal and spatial scales. These input parameters include rainfall runoff relationships, build up and wash off of total suspended solids (TSS) and various pollutants from different land use areas, and variations on multiple instream processes that may transform, degrade or otherwise alter the transport of pollutants through the watershed. The additional work will be used to better characterize runoff behavior of pollutants of concern, as well as evaluate model performance on different spatial and temporal scales. The evaluation of considerations and limitations of the model in Southern California watersheds will result in recommendations for parameterization and calibration of other models for use in Southern California.

Technology transfer. An understanding of modeling fundamentals and principles is crucial in interpreting modeling results and applying those results in a meaningful way. Effective communication between the modelers and decision makers is crucial to appropriate use and application of models. SCCWRP will facilitate communication and education of managers on modeling principles and will serve as an ongoing resource to our member agencies for model selection, use, and interpretation.

Project Status: This is the sixth year of an ongoing project. Previous years have focused on an assessment of data needs, coordination with key watershed stakeholders, data collection, and initial model development. The sixth year will focus on continued development of the water quality models for bacteria, trace metals, and nutrients. We will place particular emphasis on investigation of pollutant partitioning to various size fractions of storm water particulates. These tasks will necessitate ongoing field data collection for model calibration and validation.

Collaborators: This project is being conducted in collaboration with the Los Angeles/San Gabriel Rivers Watershed Council and Santa Monica Baykeeper and partially funded by the Los Angeles RWQCB, San Diego RWQCB, City of Los Angeles, Los Angeles County Department of Public Works, Orange County Resources and Development Management Department, Los Angeles Contaminated Sediments Task Force, Los Angeles County Sanitation District, and US EPA Region IX.



A3     DEVELOPMENT OF LINKED WATERSHED-ESTUARINE HYDRODYNAMIC AND WATER QUALITY MODELS
           Lead Investigator: E. Stein

Objectives: Estuaries serve as key ecological transition zones between terrestrial and coastal marine environments. Because of their location at the bottom of watersheds, estuaries also serve as the recipient of multiple sources of pollutant inputs including urban and agricultural runoff, industrial and power generating station discharges, treated municipal wastewater discharges, shipping and boating activities, amongst others. As a result, many estuaries have degraded water quality and contaminated sediments. While most managers recognize the linkage between pollutant inputs and impacts in estuaries, there are virtually no tools available to quantitatively link the relationship between pollutant inputs and water or sediment quality in southern California estuaries. For example, little quantitative information currently exists to determine how much of the pollutant inputs from urbanized watersheds in southern California settle in estuaries or pass directly through and into the coastal ocean.

Development of estuarine models that link pollutant inputs and water or sediment quality is one quantitative tool that would enhance our understanding of how pollutants are transported through estuaries, spatial and temporal patterns of deposition and accumulation, and seasonal flushing of accumulated compounds to the ocean. Estuarine processes, however, are very complicated. Hydrodynamic issues such as bidirectional flow (associated with tides), salinity, and depth stratification affect the physical delivery, mixing, and accumulation of pollutants. There are also numerous water quality issues including the dissolution/precipitation of pollutants transitioning from fresh to salt water, flocculation and particle size dynamics, as well as sediment deposition and resuspension. All of these factors, and more, are the reason why dynamic estuarine models have not been well developed thus far.

The goal of this project is to begin developing a model that can link watershed-based sources of pollutants and their fate and transport through an estuary to the ocean. For the past four years SCCWRP has been developing regional watershed models to simulate pollutant washoff from land surfaces and the resultant water quality in rivers and streams (see Development and Evaluation of Watershed Models). The watershed model will serve as the input of watershed-based sources of pollution to the estuarine model. Development of the estuarine model will necessitate developing an understanding of the physical, chemical and biological dynamics of southern California estuaries. The project will begin with development of models for several small, urban estuaries for which SCCWRP has previously developed watershed models. In later phases, the effort will be expanded to include other types of estuaries typically found in southern California. Once developed, managers can use these linked watershed-estuary models to assess the effect of various proposed management scenarios.

Tasks: Collection of Field Data. Model calibration and validation requires data on the physical and chemical conditions within the estuary in order to adjust model parameters and test performance of the model. Physical data collected will include dimensions and bathymetry of each estuary as well as flow velocities and water surface elevations over various tidal cycles. Additional data will include measuring temperature and conductivity at multiple locations and depths to ascertain seasonal stratification patterns. Various constituents that are associated with watershed runoff, such as suspended solids, metals, nutrients, and bacteria will be measured in the water column and sediment. Later stages of the project may include special studies, such as measuring constituent flux rates between the sediment and water column.

Development of Model(s). Field data will be used to create dynamic models that simulate flow and fate of various pollutants in southern California estuaries. Model development will occur in two steps. The first step will be to develop, calibrate, and validate models of the hydrodynamics, or movement of water through the estuary. The second step will be develop, calibrate, and validate models that simulate fate and transport of various constituents through the estuary. Validation of the model for both hydrodynamics and water quality dynamics will occur on an independent data set from model calibration.

Model Evaluation (Trial application). Sensitivity analysis will be run to determine how input parameters that affect pollutant fate and transport, such as freshwater flow, particle size distribution, and watershed loading influence model performance. These analyses will be used to fine-tune the model and identify priority areas for additional data collection. In addition, several potential scenarios will be evaluated to demonstrate the model’s utility at analyzing the effect of management measures on water and sediment quality in the estuary.

Technology Transfer. An understanding of modeling fundamentals and principles is crucial in interpreting modeling results and applying those results in a meaningful way. Effective communication between the modelers and decision makers is crucial to appropriate use and application of models. SCCWRP will facilitate communication between various estuary modeling efforts in southern California for improved sharing of data and modeling approaches. In addition, SCCWRP will promote education of managers on modeling principles and will serve as an ongoing resource to our member agencies for model selection, use, and interpretation.

Project Status: This is the second year of a four-year project. The first year focused on data collection and initial development of hydrodynamic models. During the second year, we will focus on model calibration and development of linkages between watershed and estuary hydrodynamic models for the San Gabriel and Ballona estuaries/watersheds.

Collaborators: This project is being conducted in collaboration with US Environmental Protection Agency, US Geological Survey, the Los Angeles Regional Water Quality Control Board, San Diego Regional Water Quality Control Board, Port of Los Angeles, Los Angeles Department of Water and Power, City of Los Angeles, City of San Diego, Santa Monica Bay Restoration Commission, U.S. Army Corps of Engineers, and California State Coastal Conservancy.



A4    EFFECTS OF REGIONWIDE FIRES ON DEPOSTION, RUNOFF, AND EMISSIONS TO THE SOUTHERN CALIFORNIA BIGHT
           Lead Investigators: E. Stein and K. Maruya

Objectives: Fire is a natural component of Mediterranean ecosystems such as those found in southern California. Severe burns have been shown to increase runoff and sediment generation to downstream areas. Constituents associated with the increased runoff have the potential to affect water quality in downstream receiving waters and the near-shore coastal environment. This condition may be especially problematic for streams that are already impaired.

Most research on post-fire water quality has focused on nutrient and sediment enrichment in relatively natural areas. However, post-fire runoff also has the potential to increase loadings of carbon, organic compounds, such as PAHs and trace metals. Constituent loadings may occur via several routes over a range of spatial and temporal scales. Potential routes include direct runoff, debris flows, or from atmospheric deposition of ash and subsequent runoff during storms. Investigating the magnitude and duration of fire effects in downstream and/or adjacent watersheds is critical to accounting for its influence on cumulative water quality impacts and attainment of water quality standards.

This goal of this project is to investigate the fate of water quality constituents resulting from the wildfires in the Los Padres and San Bernardino National Forests in October 2003, which presented an unique opportunity to address fire-related potential for water quality impacts. There are four objectives to this project: 1) compare fire-related loadings directly from a burned watershed to those from unburned watersheds; 2) track the attenuation of fire-related loadings to watersheds affected directly by fires using stable isotope markers and those affected indirectly by aerial deposition of ash and subsequent washoff; 3) monitor succession and recovery of wetlands and riparian plant communities in burned watersheds using newly developed remote sensing and assessment techniques; and 4) determine the effect of fire-related runoff and erosion on receiving waters via plume tracking and coastal shelf sediment deposition (see Southern California Bight Regional Monitoring: 2003).

Tasks: Selection of study sites. Paired watersheds (one burned, one unburned) of comparable size, location, and setting will be identified. Efforts will be made to identify watersheds where existing pre-fire data is available. These watersheds will be used to monitor the effects of recent fires on runoff and water quality over time.

Field data collection and analysis. Storm water flow, sediment, and water quality data will be collected from both the burned and unburned watersheds. At least four storms will be monitored over a two-year period at each site. Runoff and loading characteristics will be compared between the two watersheds over time.

Isotope Marker Analysis. Storm water and low flow water samples will be filtered as soon as possible after collection using glass fiber filters. Filtrates will be processed by solid phase extraction immediately, and the filters will be stored frozen at –20oC until extracted. Sediment samples will be homogenized, freeze-dried, and stored at –20oC until extracted. All the samples will be extracted using EPA-recommended or equivalent protocols and stringent QA/QC procedures. Compound-specific stable carbon isotopic fingerprints of PAHs and alkanes will be measured, as well as isotopic fingerprints of black and organic carbon.

Project Status: This is the fourth year of a four-year project. Initial site selection and field data collections, including multiple storm events, were accomplished in the first two years. The third year focused on initiation of the isotope marker analysis and vegetation recovery tasks. This year will focus on completion of data analysis and reporting.

Collaborators: This project is being conducted in collaboration with the US Geological Survey, the Ventura County Watershed Protection District and Environment Canada. Researchers at UCLA and CSULA are also conducting related research at our study sites.



A5     REFINEMENT OF FRESHWATER BIOASSESSMENTS IN SOUTHERN CALIFORNIA
           Lead Investigator: K. Schiff

Objectives: Freshwater biological communities represent a potentially powerful tool for evaluating the effects of discharges in southern California creeks and streams. Bioassessments integrate the effects of multiple stressors, including chemical pollutants and physical alterations in receiving waters. One value of biological assessments is that they are closer to many of the defined beneficial uses of receiving waters (i.e., aquatic life, warm water habitat, cold water habitat) than chemically derived water quality objectives.

Despite their value, bioassessments have been slow to gain popularity as a monitoring tool in southern California. A big step forward was the development of the California Stream Bioassessment Protocol (CSBP) by the California Department of Fish and Game (CDFG). The protocol outlines the steps necessary for conducting rapid bioassessments of benthic invertebrates in streams and rivers statewide. However, many regulated agencies are still unfamiliar with the techniques required and lack adequate training to use the tool efficiently. Moreover, once the CSBP are used, the assessment tools that tell managers if a site is “good” or “bad” are still in early development in southern California. Finally, there is no integration among the agencies that are conducting freshwater bioassessments in southern California watersheds. As a result, there is no capability to make larger scale assessments of biological health for streams throughout the southern California region.

The goal of this project is to refine bioassessments in southern California. The refinement will be accomplished in three main areas. First, a monitoring infrastructure will be built for those agencies that wish to use bioassessment as a monitoring tool. This infrastructure will include methods standardization, training, quality assurance, and information management. Second, assessment tools will be evaluated and refined for use throughout southern California. Third, a study design will be created that enables integration across multiple programs to answer questions of interest at large regional scales.

Tasks: Building a monitoring infrastructure. This task will develop a region-specific standard operating procedure (SOP) for bioassessment monitoring in southern California. This SOP will build off of existing bioassessment protocols established for the State by the California Department of Fish and Game and the Surface Water Ambient Monitoring Program. A training workshop will be given to assist those agencies not currently conducting bioassessments. The training workshop will be supplemented with audits of field and laboratory activities. Finally, an information management system will be developed for storage, retrieval, and sharing of bioassessment and supplemental data.

Evaluation and refinement of assessment tools. Existing assessment tools, such as the index of biological integrity (IBI), will be evaluated for use throughout the southern California region. The IBI was originally developed based largely on sites collected from high gradient, riffle-pool dominated systems with perennial flow. Many streams in southern California, particularly those potentially impacted by human development, are low gradient non-riffle systems or streams with nonperennial flow. The IBI will be evaluated using independent data sets from these two habitat types.

Develop a regional bioassessment monitoring workplan. A regional monitoring workplan will be created for assessing the status of watershed health throughout the southern California region. The workplan will define the monitoring questions of interest, the general approach to study design, and monitoring specifics including monitoring sites and frequency. The workplan will ensure integration with existing monitoring programs including local permit (NPDES) monitoring, statewide monitoring (SWAMP), and federal monitoring (EMAP) programs.

Project Status: This is the third year of a three-year study. The first year focused on building the monitoring infrastructure including training and the initiation of monitoring manuals. The second year focused on collection of additional data to support assessment tool refinement. The third year will complete the assessment and finalize the regional monitoring study design.

Collaborators: This project is being conducted in collaboration with the CDFG and is partially funded by the Stormwater Monitoring Coalition, the Los Angeles County Sanitation District, and the SWRCB Surface Water Ambient Monitoring Program.



A6     DEVELOPMENT OF BIOINDICATORS FOR EPHEMERAL STREAMS
           Lead Investigator: E. Stein

Objectives: Bioassessment methodologies are being developed for ambient monitoring of perennial streams including benthic macroinvertebrate assemblages to assess watershed health (See Refinement of Freshwater Assessments in Southern California). However, traditional benthic macroinvertebrate bioassessments may be inadequate for many southern California ephemeral or intermittent stream reaches, because they are not inundated for a long enough time to develop a mature community (approximately six weeks). This can impact the region’s ambient monitoring efforts because only about 30% of the state’s total stream miles are perennial.

An alternative bioassessment tool that has been used in freshwater streams is periphyton. Periphyton, or attached algae, can grow rapidly (i.e., two to three weeks) substantially reducing the lag time between mature assemblages and ephemeral flow duration. Diatoms, in particular, are increasingly being used as bioindicators for environmental monitoring because they reproduce quickly and are sensitive to a number of environmental stressors such as trace metals, herbicides, pH, turbidity, and salinity, amongst others.

This goal of this project is to investigate the use of periphyton as a bioindicator in streams in southern California. The investigation will include a literature review and pilot field testing of potential bioindicator(s). We will examine multiple methods for sampling attached algae, taxonomic resolution, and a variety of indices used by others in different parts of the country.

Tasks: Literature review. The bioindicator literature will be comprehensively reviewed in order to learn what bioindicators are being used elsewhere, corresponding methodologies, and whether they could be adapted to southern California ephemeral streams. The conclusions of this review will be used to identify priority research questions for bioindicator development in southern California.

Pilot testing of proposed bioindicators in ephemeral and intermittent streams. Potential methods for ephemeral and intermittent streams identified in the literature review will be identified and tested. A preliminary examination of bioindicators relative to other potential stressors (e.g., habitat, water quality, hydrology) will be conducted to begin evaluating the efficacy of selected bioindicators. The pilot testing will result in a publication on initial findings and next steps for development of periphyton as a bioindicator for southern California streams.

Project Status: This is the second year of a two-year project. The first year focused on a literature review, development of a sampling protocol, and preliminary data collection. The second year will complete data analysis and creation a conceptual assessment tool.

Collaborators: This project is being conducted in collaboration with California State University San Marcos, California Academy of Sciences, and Scripps Institute of Oceanography



A7     BIOACCUMULATION IN FISHES CONSUMED BY ANGLERS IN VENTURA AND LOS ANGELES COUNTY WATERSHEDS
          Lead Investigator: J. Allen

Objectives: One goal of the Clean Water Act is to ensure that all water bodies are “fishable and swimmable”. In southern California, many water bodies are impacted the presence of bioaccumulative substances. Regulatory agencies faced with maintaining fishable water bodies rely on a risk-based approach for setting water quality objectives. These objectives focus on the bioaccumulation potential of many contaminants from water to fish and then fish to humans. In a recent study, we assessed the types and extent of fishing (i.e., commercial, recreational, subsistence), types of species caught, and amount of fish consumed in selected rivers and streams of Ventura and Los Angeles Counties. These data provide information on what species are most frequently caught and consumed and sites or habitats where they are most consumed.

While information on fishing pressure now exists, managers lack information of the levels of bioaccumulative substances in freshwater species. An assessment of bioaccumulation in fish species known to be consumed at known sites along with consumption rates of these species can then be used by regulators and stakeholders as part of risk assessment models, or to make decisions regarding modifications of beneficial use designations for recreational or subsistence fishing.

The objective of this study is to determine contaminant concentrations of fish species most frequently caught and consumed by anglers and at sites where they are most consumed. Levels of these contaminants will be compared to human health-risk screening values for contaminants. This information will provide a basis for assessing potential effects to beneficial use of watersheds in Ventura and Los Angeles Counties.

Tasks: Collect fish for tissue analysis. The fish species most frequently caught and consumed by anglers will be collected from sites where they were found to be most frequently consumed in our recent freshwater fish consumption study. Appropriate collecting methods will be used to collect samples of these species at the sites. In addition, for species that are stocked, we will obtain fish from the sources of the stocked fish for analysis.

Laboratory processing and chemical analysis. The gender, age, and weight of each fish in a composite will be determined in the laboratory. Muscle fillets from each fish will be dissected and will be analyzed for PCBs, DDTs, other bioaccumulative substances.

Data analysis and report preparation. Tissue concentrations of PCBs, DDT, and other pesticides will be analyzed to compare tissue contamination data between sites and species, and for evaluating contamination levels relative to human health-risk screening values.

Project Status: This is the first year of a two-year study. Collection and chemical analysis of fish species will be done in the first year. The second year will focus on completing data analysis and production of a final report.

Collaborators: None at present.



A8     CHARACTERISTICS OF EFFLUENTS FROM LARGE MUNICIPAL WASTEWATER TREATMENT FACILITIES
          Lead Investigator: E. Stein

Objectives: Pollutant mass emissions from the four largest Publicly Owned Treatment Works have traditionally been the largest contaminant input to the SCB. However, contaminant loads from these sources have declined by more than 95% over the last 30 years as a result of increased effluent treatment, source control, industrial pretreatment, and reclamation. This project involves characterizing each agency’s effluent from their discharge monitoring reports and calculating mass emission estimates during 2005-2006 time period. These calculations will continue the time series of annual mass emission estimates dating back to 1972.

Tasks: Data acquisition. Data will be acquired directly from annual, semi-annual, quarterly, or monthly effluent summaries reported to Regional Water Quality Control Boards. The data acquired will include flow, laboratory methods, and constituent concentrations. These self-monitoring results will be checked for adherence to quality assurance and comparability, then entered into our mass emissions database.

Data analysis and report preparation. Data reduction will involve mass loading calculations based upon flow and concentration. The smallest time intervals available (i.e., monthly) for these parameters will be used and then summed for annual loads. Tables of mean annual concentration and total mass for 2005 and 2006 will be prepared. Report preparation will compare POTW discharges over time dating back to 1971.

Project Status: This is an ongoing project. This year, we will compile the most recent annual monitoring reports, update our existing database and investigate emerging and ongoing trends in POTW discharges.

Collaborators: This project is being conducted in collaboration with the City of Los Angeles (Environmental Monitoring Division), County Sanitation Districts of Los Angeles County, Orange County Sanitation District, and City of San Diego (Metropolitan Wastewater Department).



A9     LARGE AND SMALL SCALE DEPOSITION OF ATMOSPHERIC TRACE METALS IN SOUTHERN CALIFORNIA
           Lead Investigator: K. Schiff

Objectives: Recent research by SCCWRP and UCLA has identified that dry atmospheric deposition can be a significant source of nutrients (nitrogen), trace metals (Cr, Cu, Ni, Pb and Zn), and polynuclear aromatic hydrocarbons (PAHs) in coastal watersheds. Up to 90% or more of these loads are entrained in surface runoff from impervious surfaces during subsequent rain events. Most of this deposited material is relatively coarse (>10 microns) and much is derived from roadside dusts. Our investigation into small-scale transport of roadside dusts has shown trace metal deposition along a gradient extending downwind of freeways.

Despite the increased knowledge of small scale impacts, relatively little information exists on large-scale deposition throughout the Southern California Bight. The goal of this study is to extend our studies of deposition to new regions of the Southern California Bight in order to help define the magnitude of this environmental concern. This study will cross municipal, jurisdictional, watershed, and airshed boundaries. Large scale transects will be used to estimate atmospheric deposition from San Diego to Santa Barbara (>150 mi).

Tasks: Sampling design and coordination. The first task will focus on optimizing the sampling design to maximize efficiency. Limited resources mandate effectiveness across the multiple spatial scales being implemented in this study. This task will be critically important for success due to the cross-media, cross-jurisdictional, and cross-disciplinary scientists involved in this study.

Atmospheric deposition sampling and analysis. Sampling of atmospheric deposition for trace metals will be primarily accomplished using surrogate surfaces. Surrogate surfaces have the advantage of being quantifiable, reproducible, and inexpensive. This enables scientists to compare deposition at a number of sites all collected simultaneously enhancing the ability to test for differences among different locations. Samples collected from surrogate surfaces will be analyzed for a variety of inorganic constituents including trace metals such as chromium, copper, lead, nickel, and zinc. Because organic constituents can also occur as gas phase components in the atmosphere, sampling will include using high volume pumps and in-situ extraction devices such as polyurethane foam (PUF). Organic constituents will include PCBs, DDTs, Chlordanes and PAHs.

Integration of study components. Large-scale and small-scale spatial analysis will compare dry weather deposition flux within a watershed, across nearby watersheds, between distant watersheds, and among airsheds. Spatial gradients will be correlated with ambient atmospheric concentrations to help determine predictive relationships between these different spatial scales. For gas phase organic species, estimates of deposition relative to water column of sediment concentrations will also be made.

Project Status: This is the third year of a three-year project. Planning and pilot studies occurred in year one. Sampling was begun in year two. Field sampling and laboratory analysis will be completed in the third year and estimates of cross media flux will be calculated.

Collaborators: This project is being conducted in collaboration with the University of California Los Angeles (Dr. Keith Stolzenbach) and is partially funded by the San Diego and Los Angeles RWQCBs.



A10     COMPARISON OF MASS EMISSIONS AMONG SOURCES IN THE SOUTHERN CALIFORNIA BIGHT
           Lead Investigator: E. Stein

Objective: One tool that managers use to evaluate potential risk in the Southern California Bight (SCB) is to estimate mass emissions of constituents of concern. Managers use this tool to compare mass emissions among sources. Managers also use this tool to compare mass emissions over time from the same source to assess if discharges are increasing or decreasing in magnitude relative to other sources. In addition, managers can use this data to assess cumulative impacts in the SCB though the use of a mass balance model. Estimates of historical mass emissions can be compared to estimates of contaminant mass presently residing in the SCB developed as part of the SCB regional monitoring program (see 2003 REGIONAL MONITORING SURVEY).

SCCWRP has conducted mass emissions from a variety of sources at periodic intervals dating back to 1970. Estimates of mass emissions from large publicly owned treatment works (POTWs) have been conducted annually for the last 30 years. Estimates from other sources, such as small POTWs, industrial dischargers, dredged material disposal, urban runoff, oil platforms, vessel discharges, and aerial deposition have been conducted at a lesser frequency, but approximately every five years. SCCWRP’s last effort to characterize all sources occurred in 2000. The goal of this project is to once again estimate mass emissions from all sources for the 2005-2006 time period to: (1) assess the total mass emissions to the Southern California Bight; (2) determine the relative contribution of each source; and (3) evaluate trends in mass emissions from each source over the last 30 years.

Tasks: Data acquisition. Data will be acquired directly from annual, semi-annual, quarterly, or monthly effluent summaries reported to Regional Water Quality Control Boards. The data acquired will include flow, laboratory methods, and constituent concentrations. These self-monitoring results will be checked for adherence to quality assurance and comparability, then entered into our mass emissions database. Some sources are not monitored under an NPDES permit. These sources will be evaluated independently.

Data reduction and report preparation. Data reduction will involve mass loading calculations including estimates of uncertainty. These estimates will be prepared for each of the targeted sources. For the monitored point sources, we will calculate these estimates based upon flow and concentration. The smallest time intervals available (i.e., monthly) will be used and then summed for annual loads. Tables of mean annual concentration and total mass for the 2005-06 time period will be prepared. Report preparation will compare mass emissions among sources and assess changes in emissions over time from 1971 to 2005.

Project Status: This is an ongoing project. This year, we will compile discharge data from all sources over the past five years and assess the relative contribution of each source to total emissions to the SCB. In addition, we will begin the process of updating our mass emission database with 2005-06 discharge data for small POTWs and industrial dischargers.

Collaborators: There currently are no collaborators for this project.




WETLANDS AND ESTUARIES


B1     HISTORIC ECOLOGY OF SOUTHERN CALIFORNIA'S COASTAL WATERSHED AND WETLANDS
          Lead Investigator: E. Stein and M. Sutula

Objectives: As pasturelands have been converted to cities, streams have been dammed, channelized, and diverted for water conservation and flood control purposes over the last 200 years, Southern California’s coastal watersheds have undergone dramatic transformations. In reaction to the highly altered nature of southern California’s urban watersheds, numerous watershed plans are currently being developed that include proposals to enhance and restore streams and wetlands to improve habitat quality, recreation opportunities, and water quality.

For restoration plans to be successful they must be based on an understanding of natural baseline or reference conditions, including the historic extent and condition of streams and floodplain wetlands. Currently, this information is not readily accessible to environmental managers, scientists, and the public, but it is vitally important for answering a range of key questions about the restoration potential of the watershed. Examples of key questions include where to restore streams, recommended native vegetation landscaping palette, effect of major stressors, and context for development of monitoring and assessment programs.

This project will begin filling the data gap on baseline conditions by examining the historic extent and distribution of wetlands in the San Gabriel Watershed and several proximate coastal estuaries. The objectives of this project are to develop a framework and infrastructure for compiling sentinel data sets on historic condition, and to use these data to evaluate how the distribution of wetlands have changed over time in response to key changes in land use or stream management.

Tasks: Develop historical data archive and database. A collection of existing studies, documents, maps, and related information pertaining to historical conditions, will be developed to serve as the foundation for an authoritative historical ecological analysis in the Project Area. This will result in a bibliography cataloging historical data sources, and substantial archives housed at a selected site in the watershed and at USC/Green Visions Project. The data collection phase will also contribute to public awareness and understanding of the project.

Develop historical land use maps and timeline. The ecological and geomorphic processes presently shaping the San Gabriel River watershed represent a combination of both natural factors and human activities. A functional understanding of the system requires knowledge about the specific history of land use activities and natural events (e.g., watershed-scale floods and fires) that have significantly impacted overall system function. In this task, we will identify both the time period and spatial extent of major management activities, including clearing, grazing, channelization, mining, and others, concentrating on the period of 1890-1930, but also providing land use change information through present day.

Digitize and georectify key historical data sets. Some of the most important historical data sources exhibit a high degree of spatial accuracy and thus have the potential to be directly useful for local science and planning. For example, the mapping of wetland features in 19th-century US Coast Survey maps can have a greater level of horizontal control than many standard modern maps (Grossinger 1995). In this task, we will create high-resolution digital scans and use several tested approaches to accurately georectify the selected historical maps and early aerial photography to modern coordinates. These products will provide the basis for GIS mapping in this project and will also have much broader utility for a wide range of applied uses including environmental management, agricultural management, and operations and maintenance. The digital scans will also provide a wealth of images for use by local resource managers, environmental organizations, and educators

Map historical wetlands and riparian areas. In this task, relevant priority data sources will be used to map wetland and riparian habitat present in the Project Area during key index periods. The habitats will be mapped using methods and classification systems that facilitate comparison with contemporary NWI maps to the extent possible given limitations in the historic data. These maps will be used to provide a landscape profile of wetland and riparian habitat distribution for the Project Area. A landscape profile is a term used to describe how the habitats are distributed across the watershed or geographic areas of interest. The results of an inventory are displayed by histograms or tables approximate area by categories of interest, such as subcatchment, political boundaries, stream order, habitat type, etc.

Research distribution of riparian vegetation community types and/or species. This task will provide information on the spatial distribution of riparian vegetation community types and species on representative geomorphic sections of the Project Area.

Communication and Outreach. The purpose of this task is to communicate the results of the project to stakeholder groups and the interested public through presentations and a final project report.

Project Status: This is the first year of a two-year project.

Collaborators: This project is being conducted in collaboration with the University of Southern California, California State University Northridge, San Francisco Estuary Institute, and the Los Angeles and San Gabriel River Watershed Council. The project is funded by grants from the Rivers and Mountains Conservancy and from USC Sea Grant.



B2     TECHNICAL SUPPORT FOR DEVELOPMENT OF NUTRIENT CRITERIA FOR COASTAL ESTUARIES AND LAGOONS
           Lead Investigator: M. Sutula

Objectives: The US EPA is currently working with the State Water Resources Control Board to develop nutrient criteria that can be used as endpoints to protect the State’s waters from nutrient over enrichment. Nutrient criteria must be placed within the context of the Clean Water Action Plan to meet its primary goal of maintaining nutrient levels that support the health of aquatic systems and limit the excessive growth of macrophytes or phytoplankton, potentially harmful algal blooms leading to oxygen declines, imbalance of aquatic species, public health threats, and a general decline in aquatic resources.

The EPA Region IX nutrient criteria development program was initiated in 1999 and has focused primarily on fresh water systems including streams, rivers, lakes, and reservoirs. While estuaries and coastal lagoons are an important resource, less progress has been made on developing a conceptual approach and defining nutrient criteria for these waterbodies.

The goals of this project are to provide technical support for the development of nutrient criteria in estuaries and lagoons. Specific objectives include: 1) develop a conceptual model for estuarine nutrient criteria development, 2) identify current research on nutrient related issues in southern California estuaries, 3) identify data gaps, and 4) draft a research plan to address those data gaps. Ultimately, these projects will lead to a multi-disciplinary research program with the aim of developing quantitative numeric nutrient criteria.

Tasks: Review Research on Nutrient Cycling in California Estuaries. Literature will be reviewed and a conceptual model will be developed that illustrates estuarine nutrient cycle pathways. This conceptual model will be used as a tool to identify potential secondary indicators of estuarine eutrophication. These indicators will provide the basis for the types of research and data that will be gathered for this task. SCCWRP will provide input on the conceptual model and classification system for estuaries. Based on the indicators illustrated in the conceptual model, SCCWRP will identify estuarine water quality and watershed data sources for each estuary located along Southern California’s Coast within the jurisdiction of Regional Boards 4, 8, and 9. Metadata on existing data sources will be collected using a 3-tiered, hierarchical approach. These three tiers are (1) existing monitoring data, (2) data from on-going projects, and (3) data from special studies. Potential data sources could include two types of data: “hard” data from water bodies as well as “soft” data acquired via modeling scenarios. Additionally, geospatial topographic and watershed land cover data will also be compiled.

Develop Estuarine Nutrient Criteria Research Plan. Data from Task 1 will be reviewed to identify data gaps and key uncertainties, as well as to refine the initial risk models and endpoints to make them more relevant to linking nutrient loads and use impairment. An estuarine nutrient criteria research plan will be developed to address these data gaps.

Project Status: This is first year of a one-year project.

Collaborators: This work is being conducted in collaboration with the TetraTech Inc. and is partially funded by US EPA Region IX.



B3     RELATIONSHIPS BETWEEN DISSOLVED OXYGEN AND ALGAE DISTRIBUTION IN NEWPORT BAY
           Lead Investigator: E. Stein

Objectives: Newport Bay is the second largest estuarine embayment in southern California and provides critical natural habitat for terrestrial and aquatic species. The Lower Bay is a regionally important recreational area, with one of the largest pleasure craft harbors in the United States. The upper portion of the Bay is a state ecological reserve and provides refuge, foraging areas, and breeding grounds for a number of threatened and endangered species. The Bay is also a significant spawning and nursery habitat for commercial and non-commercial fish species. However, beneficial uses of the Bay are threatened by numerous sources of pollutant loading either directly, or by tributaries of its watershed. The watershed is rapidly converting from orchards and farms to an urban environment. High nutrient loads from the surrounding watershed have resulted in excessive growth of macroalgae (Enteromorpha and Ulva spp.) and impairment of the Bay’s designated beneficial uses. As a result, the RWQCB adopted a Total Maximum Daily Load (TMDL) for the Bay for nitrogen (N) and phosphorus (P) in 1998.

The large macroalgal blooms resulting from nutrient over-enrichment often adversely impact a number of aquatic system beneficial uses through reduction in water column dissolved oxygen (DO) concentrations. To help protect beneficial uses, the spatial and temporal extent of hypoxic/anoxic events needs to be better understood and the link between algal blooms and reductions in DO levels in the Bay needs to be investigated. Monitoring of DO in the Bay to date has been insufficient to determine these linkages.

The goal of this study is to characterize the DO and macroalgae regimes of Upper Newport Bay (UNB). First, the spatial and temporal extent of hypoxia/anoxia in UNB will be determined through continuous monitoring of DO in surface and bottom waters. Second, macroalgal abundance will be estimated using remote sensing techniques during the period of DO sensor deployment to determine if there is a quantitative relationship between intertidal macroalgal abundance and the frequency of hypoxic events. The estimated abundance of macroalgae at points closest to the DO sensors will be used to help interpret the observed patterns in DO and any hypoxic episodes that occur. This interpretation will assist the Santa Ana RWQCB in development of DO criteria as a water quality management tool.

Tasks: Continuous monitoring of dissolved oxygen. Three buoys, each equipped with two multi-parameter sondes, will be deployed in UNB from May through December 2005 to determine the spatial and temporal extent of hypoxia in UNB (weather permitting). The sondes will measure DO, pH, temperature and conductivity continuously at 30 minute intervals at both the surface and near the bottom of the bay.

Macroalgal bloom time series remote sensing. To determine if there is a quantitative relationship between intertidal macroalgal abundance and the frequency of hypoxic events, macroalgal abundance will be estimated using aerial imaging techniques during the period of deployment of DO sensors in UNB. Quantitative field measurements of macroalgal abundance will be conducted coincident with the remote sensing. The in Bay data will serve as the true value to which the estimates made from aerial images will be compared.

Data analysis and reporting. Dissolved oxygen data will be analyzed for seasonal, diurnal, tidal, and spatial (lateral and vertical) patterns. Overall macroalgal abundance in discrete areas near sondes will be used in regression analysis to determine if there is a reliable, predictable relationship between macroalgal biomass and the occurrence of hypoxia. A report will be drafted summarizing the spatial and temporal patterns in DO and macroalgae and discussing the utility of using remote-sensing to measure macroalgal abundance.

Project Status: This is the second year of a two-year project. The first year focused on collection of field data and aerial images. The second year will complete data analysis and image interpretation.

Collaborators: This project is being conducted in collaboration with the Orange County Resources and Development Management Department, Irvine Ranch Water District and Moss Landing Marine Laboratories (Dr. Krista Kamer). This project is partially funded with a Proposition 13 grant to the County of Orange.



B4     SOURCE APPORTIONMENT OF PESTICIDES IN THE UPPER NEWPORT BAY WATERSHED
          Lead Investigator: K. Maruya

Objectives: Upper Newport Bay (UNB), similar to many estuaries, contains sediments contaminated with a variety of chlorinated and organophosphorous pesticides including chlorpyrifos, diazinon, chlordane, oxychlordane, dieldrin, DDT, and toxaphene. The problem is especially of concern in UNB because this estuary is home to several rare or endangered species that may become a receptor for these toxic and/or bioaccumulative contaminants. Unfortunately, the sources for many of these pesticides are still unclear and this makes implementation of management actions to control and reduce sediment concentrations in UNB difficult.

The primary constraints that limit UNB managers from quantifying sources of the target pesticides are technological. First, many constituents are found at low levels, making measurement of these constituents difficult and costly. Second, many target constituents are subject to widespread use. For example, the organophosphate pesticides have commercial, agricultural, and homeowner applications. Third, several of the target constituents are legacy pesticides where the sources are no longer application, but vectors of redistribution. The sources may not be specific land uses or businesses, but instead may be transport mechanisms such as atmospheric deposition, contributions from erosional processes, or resuspension of bed sediments.

This project will employ two relatively new analytical methods to help identify the sources and fates of the targeted chlorinated and organophosphorous pesticides in UNB. The two new techniques are chiral gas chromatography (CGC) and compound-specific isotope analysis (CSIA), which can provide new information that conventional methods are incapable of obtaining. Both CGC and CSIA will be applied to samples of wet and dry weather flows into UNB and atmospheric concentrations over UNB to generate enantiomeric fractions (EFs) of alpha- and gamma-chlordanes, oxychlordane, o,p’-DDT and DDD and selected toxaphenes, and the compound-specific stable carbon isotope ratios (IRs) of the target analytes. The EFs and IR fingerprints will be used to apportion the above-indicated transport mechanisms into various source categories for pesticide inputs, such as discharge from agricultural, commercial or household uses, resuspension of historically deposited pesticide-containing sediments, and long-range transport via the atmosphere.

Tasks: Determination of pollutant concentrations in stormwater and receiving water samples. Dissolved and suspended particulate phase samples during wet weather storm events will be collected and analyzed for TMDL regulated organic pollutants (DDTs, chlordanes) using GC-ECD and GC-MS. Following the wet season, bed sediments in the Bay at the mouth of the river will also be analyzed.

Contaminant source tracking and modeling. Organic pollutants that are elevated in concentration (e.g., p,p’-DDE) or exhibit chirality (e.g., o,p’-DDT, pyrethroids) will be analyzed by GC-C-IRMS] to quantify stable isotopic and by enantioselective GC to determine EFs. These measurements will be compared among stations and incorporated into a source apportionment model.

Project Status: This is the third year of a three-year study. The first and second years focused on field sampling and laboratory analysis. The third year will complete these activities and focus on reporting.

Collaborators: This project is being conducted in collaboration with University of California, Riverside (Dr. Jay Gan) and is partially funded by a joint State Water Resources Control Board/Department of Pesticide Regulation PRISM grant.



B5     INVESTIGATION OF CONTAMINANTS IN THE UPPER NEWPORT BAY Picivorous FOOD WEB
          Lead Investigator: J. Allen

Objectives: Newport Bay is the largest wetland in Orange County and represents an important habitat for resident and migratory birds, including several threatened or endangered species (e.g., brown pelican, least tern, and osprey). It is also an important site for recreational fishing. Despite its ecological and recreational importance, portions of the bay are contaminated by chlorinated pesticides (DDTs, chlordane, dieldrin), polychlorinated biphenyls (PCBs), and trace metals. Elevated levels of DDT and PCB may also pose cancer risks to humans.

As a result of the potential water quality impairments from these contaminants, regulators have prompted the development of a Total Maximum Daily Load (TMDL) for this water body. The many species of birds that nest or feed in Newport Bay are important receptors for contaminants. A key step in estimating contaminant exposure risk to birds is to determine contaminants in their diet. Dietary uptake can be one of the major sources of exposure for wildlife and humans, particularly for bioaccumulative compounds like DDTs, PCBs, and selenium. Recent SCCWRP research has shown that most fishes examined in the Bay had levels of DDT above wildlife health-risk guidelines, many had elevated levels of PCB, and some had elevated levels of selenium.

The objective of this study is to assess the transfer of organochlorines and trace metals in food chain pathways in Newport Bay leading to threatened/endangered bird species or humans. We will measure several components of the diet for both pathways, focusing on invertebrates eaten by fish that are eaten by birds. Understanding the pathways of bioaccumulation will help managers assess the risk of these constituents in Newport Bay. In addition, we hope to identify fish species that could be used as surrogates for assessing ambient water quality relative to wildlife and human protection.

Tasks: Sampling and analysis of fish consumption. Samples of fish will be collected using beach seine and then their stomach contents will be examined. Stomach content analysis will be used to prioritize which prey species will need to be sampled and analyzed

Conduct chemical analysis of fish tissue samples. This task focuses on chemical analysis of composite samples of whole fish species that are eaten by piscivorous bird species in Newport Bay. Analysis will focus on PCBs, pesticides, and trace metals that may be detrimental to higher order predators.

Conduct chemical analysis of fish prey and sediment samples. Important prey items of fish species consumed by birds of concern as well as sediments will be analyzed for the same PCBs, pesticides, and trace metals found in the fish species.

Data analysis and reporting. Data will be analyzed to assess the potential flow of contaminants from sediments, to fish prey, to fish, to birds of concern. Descriptions of contaminant levels in separate trophic levels and several foodweb pathways leading to birds of ecological concern will be conducted. Ultimately, the data analysis will identify fish species that could be used as surrogates for assessing ambient water quality relative to wildlife protection.

Project Status: This is the third year of a three-year study. The first year focused on sampling logistics and production of a quality assurance plan. The second year focused on collection of fish for tissue analysis, fish stomach content analysis, and collection of fish prey and sediments. Chemical analysis of fish tissue, food organisms, and sediment was also begun in this year. The third year will complete chemical analysis of existing samples, data will be analyzed, and a draft and final report will be produced.

Collaborators: This project is being conducted in collaboration with California State University, Long Beach (Dr. Zed Mason) and University of California, Riverside (Dr. Daniel Schlenk), and Santa Ana Regional Water Quality Control Board. It is partially funded by the State Water Resources Control Board through a Proposition 13 grant.



B6     EFFECTIVENESS OF TREATMENT WETLANDS AS STORMWATER BMPS AND COMPATIBILITY WITH WILDLIFE BENEFICIAL USES
          Lead Investigator: M. Sutula

Objectives: With the increasing urbanization of coastal watersheds throughout southern California, discharges of nonpoint source pollution has increased dramatically. Requirements for municipal control of runoff quantity and quality have created a demand for wetlands as effective, low-cost best management practices (BMPs) to improve surface water quality and attenuate storm flows. Concurrent with this need, wetlands and riparian areas have been rapidly disappearing from the landscape, and those that remain are often highly degraded. As a result, there is increasing pressure to restore, enhance and create natural or artificial wetlands with a goal to offset loss of habitat.

As a result of a demand for water quality treatment and the need for habitat restoration, wetland projects are being proposed with multiple objectives. State and Federal agencies are soliciting proposals (i.e., Proposition 12, 13, 40, and 50 funds) to create wetlands that will support habitat, treat nonpoint sources of pollution, attenuate floods, and create recreational opportunities. However, there is virtually no information on the compatibility of these multiple uses. Scientists and managers alike are unsure if these proposals to use wetlands will comply with requirements of their Nonpoint Source goals or NPDES permits. As a result, there is an increasing need for more information on: 1) effectiveness of treatment wetlands as a BMP for urban runoff, 2) circumstances where wetland BMPs for urban runoff are not compatible with wildlife beneficial uses, 3) modifications to siting, design or management criteria that will mitigate adverse impacts to wildlife, and 4) minimum recommended criteria to monitor treatment effectiveness and impacts to wildlife.

The goal of this project is to evaluate the effectiveness of wetlands for use as urban runoff BMPs, and their compatibility with wildlife beneficial uses in Southern California. Treatment wetland effectiveness and compatibility with wildlife beneficial use will be assessed in several stages. First, current and future planned projects will be inventoried and all relevant technical and project monitoring data will be compiled. Second, a baseline habitat survey will be conducted to evaluate wildlife beneficial use in a variety of treatment wetland system types. Results of the project evaluation and baseline habitat survey will be used to recommend interim guidance on minimal monitoring criteria and siting, design and management criteria. These criteria will help to improve BMP effectiveness and mitigate risk to wildlife. Third, a detailed study of treatment wetland effectiveness and compatibility with wildlife beneficial use will be conducted for at least one class/type of treatment wetland.

Tasks: Produce an inventory and GIS database of water quality wetlands in project area. This task will define “water quality wetland” projects to be included in the inventory, conduct a census of water quality wetland projects in the study area, and develop a GIS-compatible database of water quality wetland projects. Inventory data will consist of project technical data such as project type, size of treatment area, size of catchment basin, and treatment objectives. Monitoring data for the projects will be reviewed and a summary of each project’s monitoring program will be made, including indicators, sampling design, frequency, hydraulic and contaminant loading rates, treatment effectiveness, and impacts on or use by wildlife. In addition, project documentation will be reviewed and, where feasible, interviews with treatment system personnel will be conducted in order to review siting and design specifications, and maintenance procedures that can influence ability of treatment wetland to improve water quality and support wildlife.

Baseline Treatment Wetland Habitat Survey. The purpose of the habitat survey is to describe baseline biological conditions of sampling activities will describe baseline biological condition. Tier I sampling, which consists of habitat mapping and evaluation of general habitat condition using a rapid assessment method, will be used to describe the range and general condition of habitats found at 30-40 treatment wetlands and their reference sites. Tier II sampling represents more intensive evaluation of habitat condition and will be conducted on a subset of the sites (approximately 24 sites). Indicators of habitat condition used in this intensive survey include plant community composition, composition of benthic macroinvertebrate (BMI), fish, bird and herpetofaunal communities, and analysis of sediments, BMI and fish tissues for contaminants. This information will be used to evaluate the quality of habitat as well as the overall risk to wildlife that utilize these sites. In addition, it will also serve as the basis to prioritize a set of research questions that will drive the focus of a more intensive research study in the third task.

Study of treatment effectiveness and habitat value in a representative treatment systems. Based on the survey of wetland types/classes, targeted sampling will be conducted at a limited number of sites to determine treatment effectiveness and evaluation of wildlife beneficial use. It will assess important elements quantifying the magnitude of risk to wildlife relative to treatment efficiency and habitat value. Equally important, this task will help identify specific monitoring questions and designs for monitoring all treatment wetlands relative to wildlife risk.

Project Status: This is the third year of a four-year project. The first two years focused on compiling the inventory/database of treatment wetlands in southern California and designing the detailed sampling program with the project’s technical advisory committee. The third year will evaluate selected sites in terms of habitat quality, effectiveness, and potential ecological risk.

Collaborators: This project is being conducted in collaboration with the Los Angeles, Santa Ana and San Diego Regional Water Quality Control Boards, the California State Coastal Conservancy, and member agencies from the Southern California Wetland Recovery Project. This project is partially funded by the State Water Resources Control Board through a Proposition 13 grant.



B7     REGIONAL MONITORING/ASSESSMENT PROGRAM FOR SOUTHERN CALIFORNIA WETLANDS
          Lead Investigators: E. Stein and M. Sutula

Objectives: Current approaches to the assessment of wetland condition in southern California focus on site-specific questions, usually to address permit requirements or status of site-specific restoration work. These site-specific efforts are poorly coordinated and the absence of a consistent evaluation methodology precludes comparisons among sites or assessment of regional wetland condition.

The Southern California Wetlands Recovery Project (WRP) Science Advisory Panel has endorsed the need for a comprehensive southern California regional monitoring program to address these problems. They have suggested that implementation of a comprehensive regional monitoring program for southern California wetlands should include several components including: 1) assessment of ambient conditions of a range of wetland classes; 2) evaluation of progress towards regional restoration and ecosystem recovery goals; and 3) assess the effect of anthropogenic stressors that constrain recovery of individual wetlands or portions of entire watersheds.

The first step in achieving these objectives is to update and expand the existing inventory of wetland and riparian resources in Southern California. The second step is to develop an easy to use, cost-effective tool for site-specific wetland assessments that will complement landscape-scale assessment approaches. The development of a standardized methodology to evaluate wetland ecosystem structure and function would facilitate regional assessments and would streamline reporting of monitoring data, making them more accessible for routine evaluation of restoration and management techniques.

The goal of this project is to develop tools and initiate the development of a comprehensive regional monitoring program for southern California wetlands. There are two major objectives: 1) update and expand present-day and historical wetland inventories, making data layers GIS-compatible, incorporating additional data such as surrounding land use and infrastructure to allow analysis of anthropogenic stressors on wetland condition; 2) develop and verify rapid assessment methods to cost-effectively assess wetland condition as a function of anthropogenic stress and restoration progress; and 3) develop information management tools and regional capacity to track the net change in wetland acreage by habitat type from wetland projects (hereto referred as project tracking).

Tasks: Update/create present-day and historic inventories of wetland and riparian resources. SCCWRP staff will collaborate with the U.S. Fish and Wildlife Service, National Wetlands Inventory (NWI) to create resource maps based on the Cowardin classification scheme. Additional attributes describing the hydrogeomorphic setting will be developed for use by NWI in their habitat mapping. An inventory of historic wetland acreage will also be established using U.S. Coast Survey maps (1850-1900). The historic wetland acreage will also be delineated, to the extent practicable, by habitat type. This information will be utilized by the WRP to guide regional restoration priorities identified in this assessment.

Develop and verify a rapid field methodology (CRAM) for assessment of wetland condition. The purpose of this task is to develop the California Rapid Assessment Method (CRAM) to assess the condition of wetlands for use in a regional monitoring and assessment program. The method will be designed so that it can identify major stressors to the resource, evaluate ambient conditions, evaluate progress of restoration for multiple wetland classes throughout the State of California. Field metrics will be selected and scaled to address hydrology, biotic and abiotic structure. A provisional assessment will be completed to verify the draft CRAM.

Calibrate the CRAM for at least two wetland classes. The field verified version of CRAM will be calibrated for at least two priority wetland classes. Calibration will consist of collecting or compiling quantitative field data on wetland function or condition that can be used to develop scaling for CRAM metrics and attributes. Additionally, we will develop a consistent database for use by CRAM teams in southern California as well as other parts of the state for compilation and analysis of statewide data on wetland conditions.

Develop information management tools and regional capacity to for wetland project tracking. One central component of the WRP Integrated Regional Wetland Assessment Program is the evaluation of the net change in wetland acreage by habitat type. This evaluation is accomplished by 1) periodic region-wide updates in wetland and riparian habitat inventories and 2) tracking of projects (restoration, mitigation, etc.) that impact wetland habitat. This task consists of coordinating with state and federal agencies to create a process for update of Tracker databases through agency funding or permit conditions and developing data forms and information management infrastructure to implement project tracking in the region.

Project Status: This is the fifth year of a five-year project. The draft CRAM was developed and field verified during the first two years of this project. During the third and fourth years we calibrated CRAM for two wetland classes, initiated several demonstration projects, and initiated development of several information management tools. The fifth year will focus on completing the demonstration/pilot projects, developing a “landscape development (stressor) index, and coordinating with federal, state, and local agencies to develop strategies and tools for future implementation of CRAM. Development of appropriate information management vehicles to initiate project tracking activities in the region will also be completed during this year.

Collaborators: This project is being conducted in collaboration with the Southern California Wetland Recovery Project, California State Coastal Conservancy, San Francisco Estuary Institute (SFEI), California Coastal Commission, Moss Landing Marine Laboratory, and the US EPA – Region IX and Office of Research and Development and is funded by the EPA Office of Water.



B8    LANDSCAPE-SCALE ASSESSMENT OF SOUTHERN CALIFORNIA RIPARIAN ECOSYSTEMS CONDITION
           Lead Investigator: M. Sutula

Objectives: Southern California coastal wetlands and watersheds have been dramatically altered or destroyed by human activities over the past 150 years, with riparian habitat reduced by 90-95%. Development pressure in coastal wetlands and watersheds continues to be intense, with a doubling of the 1995 population expected by 2020. Preservation and restoration of the remaining riparian habitat is critical for ecosystem management. However, with limited funding available for restoration, it is imperative that regional priorities are established to maximize the benefit of the resources expended on selected restoration projects

To set priorities for the preservation and restoration of riparian habitat, managers need to assess the location and relative condition of the remaining habitat. One reason this has not occurred already is because the variety, size, and extensive geographic scope of riparian areas in Southern California necessitates a tool that allows relatively rapid evaluation of the general condition of riparian resources at a broad spatial scale. Remote sensing and geographic information system (GIS) technologies offer attractive options for achieving this goal in a more efficient manner than traditional “on-the-ground” assessments. These landscape-scale assessment techniques have the potential to be both fast and accurate for the needs of riparian ecosystem managers.

The objectives of this project are to: 1) develop the methodology to perform a landscape-scale assessment of ecological integrity of southern California coastal riparian resources, 2) evaluate the relative cost and benefit of using existing land use cover versus higher resolution (e.g., IKONOS, Quickbird) data to map riparian vegetation; and 3) apply these landscape-scale assessment techniques in five pilot watersheds in southern California. This work will form the scientific foundation for a decision support tool to identify riparian conservation and restoration priorities by the Southern California Wetland Recovery Project (WRP).

Tasks: Methods development. A methodology for conducting landscape-scale assessment of riparian ecological integrity at southern California spatial scales will be developed. Method development includes two steps: 1) development of assessment framework including selection of indicators and development of metrics to measure them; and 2) computer programming of assessment framework metrics to conduct analysis in five watersheds.

Evaluation of relative cost and benefit of remote sensing technologies for riparian mapping. This task will first acquire and process high-resolution (e.g., IKONOS, EMERGE) remote sensing data to map riparian vegetation a pilot watershed. Next, the cost and utility of using this high resolution data will be compared to using existing land use cover to map riparian vegetation. The comparison will be used to formulate a strategy to map riparian resources at a regional scales and will be coordinated with statewide efforts to generate wetland and riparian maps for the State of California.

Field evaluation. Application and evaluation of the landscape-scale assessment methodology will be conducted in five pilot watersheds (one from each of the five coastal southern California counties). Field verification of the assessment methodology will include comparison of results with more intensive assessments conducted during watershed planning feasibility studies (where available), and consultation with local experts. The results of the field evaluation will be used to refine the assessment methodology.

Reporting. Following the field verification exercise, a report will be prepared describing the rationale, structure, and application of the methodology and summarizing the results of the pilot application. Results will also be made available in a GIS format via the SCCWRP web site.

Project Status: This is the fourth year of a four-year project. Method development was completed during the first year. The second and third years focused on compilation of the remaining source data sets and the method was tested/refined in five southern California pilot watersheds. Final verification, pilot testing and reporting will be completed in the fourth year.

Collaborators: This work is being conducted in collaboration with the Southern California Wetland Recovery Project, the California State Coastal Conservancy and the Conception Coast Project, with funding from the NOAA Coastal Services Center.



B9    SOUTHERN CALIFORNIA WETLAND RECOVERY PROJECT SCIENCE ADVISORY PANEL
          Lead Investigators: E. Stein

Objectives: Southern California has experienced one of the highest proportional loss of wetlands relative to any state in the country. Both the Federal and State governments have expressed goals of short-term, no-net loss and long-term, net gain of wetlands. However, coordination of a comprehensive wetland protection and recovery strategy in California has been hindered by the fact that 17 Federal and State agencies share jurisdiction and responsibility for wetland stewardship, leading to administrative and bureaucratic challenges.

In 1997, the 17 Federal and State wetland management agencies formed the Southern California Wetland Recovery Project (WRP) with a goal of increasing regional coordination of wetland preservation, restoration and management. The WRP is now a partnership of Federal and State agencies working in concert with local government, environmental organizations, and scientists to develop and implement a comprehensive plan for preserving and restoring the region’s wetlands. The WRP consists of a Board of Governors and three standing committees: The Wetlands Managers Group (WMG) is responsible for drafting the regional restoration plan and advising the Governing Board on regional acquisition, restoration, and enhancement priorities; the Public Advisory Committee that represents community and interest group views to the Governing Board; and the Science Advisory Panel (SAP) that ensures that the best available science is incorporated into the decision-making processes of the WRP.

At the request of Board of Governors, SCCWRP staff provides technical assistance to the Science Advisory Panel, with the ultimate goal of improving the regional planning of wetland conservation, restoration, and management in southern California. SCCWRP staff provide technical assistance to the WRP by 1) developing and administering an extramurally-funded research program on the constraints to wetland restoration in Southern California, 2) procuring funding and technical assistance to implement the development of condition assessment, decision support, and other tools to aid the WRP in prioritizing and evaluating preservation and restoration projects, and 3) procuring funding and provide technical assistance to develop a regional wetlands monitoring program.

Tasks: Assist in the development of a region-wide WRP monitoring program. Development of a monitoring program is a logical extension of the goal development process, as monitoring should help quantify progress towards achieving goals. This process, carried out in conjunction with SCCWRP’s parallel project Integrated Wetland Regional Assessment Program, will include updating resource inventories, developing and validating landscape assessment and rapid assessment methodologies, guiding the selection of appropriate monitoring indicators, and exploring partnerships with other organizations interested in monitoring Southern California wetlands. As a part of this process, SCCWRP staff will collaborate on a WRP Science Panel position paper outlining in detail the conceptual framework for a comprehensive wetlands monitoring program.

Assist in the development of decision support tools for wetland preservation and restoration. SCCWRP staff is currently working with NOAA Coastal Services Center (CSC), the WRP Managers Group, and WRP Task Forces to develop a decision support tool for prioritizing funding of preservation and restoration activities in riparian corridors. As part of this process, SCCWRP will work to procure funding and facilitate the identification of data gaps and the development of the data infrastructure necessary to support project implementation.

Meeting support. This task includes conducting literature searches, preparing advisory reports, facilitating coordination among SAP members, and serving as a liaison between the SAP and the WRP Managers group.

Project Status: This is an ongoing project. Activities in previous years has included development of wetland regional monitoring and assessment tools, creating a plan for implementing the suite of tools for regional assessments of estuaries, and support for the Ballona Wetlands restoration effort. This year, activities will focus on a riverine wetlands regional assessment plan, developing detailed monitoring protocols and implementation strategy for regional estuarine assessment, and continued support of the Ballona wetlands restoration.

Collaborators: This project is being conducted in collaboration with the Southern California Wetland Recovery Project and its 17 member agencies.

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COASTAL OCEAN

C1     Development of SEDIMENT QUALITY OBJECTIVES FOR BAYS AND ESTUARIES
          Lead Investigator: S. Bay

Objectives: Environmental managers often need to evaluate the significance of sediment contamination as part of water quality assessments, dredged material evaluations, or sediment cleanup activities. Many managers use sediment quality guidelines for these activities, which traditionally have been chemical specific values that try to predict the biological significance of sediment contaminant concentrations. A variety of sediment quality guidelines have been developed by various federal agencies and other states, but these guidelines differ from one another in the methods and data used to generate the values. In some applications, multiple lines of evidence, including biology, toxicology and chemistry, are used for sediment quality assessments. In the end, no consistent policy exists for the use of sediment quality guidelines for the assessment and regulation of sediments. As a result, the application of sediment quality guidelines is used inconsistently for environmental management decision-making.

The State of California intends to develop sediment quality objectives (SQOs) as an assessment tool for sediment contamination. The SQOs will include narrative descriptions of the condition to be protected and the associated analytical methods needed to determine whether the condition has been attained. The State Water Resources Control Board (SWRCB) issued a plan in 1991 for the development of SQOs, but this workplan was never implemented. The SWRCB has renewed its pledge to develop SQOs and are committed to developing and incorporating SQOs into SWRCB policy. Because of advances in methods to assess sediment quality and an improved understanding of the relationship between chemical contamination and biological effects, the 1991 SQO workplan is no longer appropriate. A SQO development program is needed that reflects the current requirements of environmental managers in California, utilizes improved techniques for sediment quality assessment, and is applicable to local contamination patterns.

The goal of this project is to conduct the technical studies necessary for development and implementation of SQOs for California’s enclosed bays and estuaries. The project consists of four major elements. The objective of the first element is to evaluate the relationships between contamination and direct effects on the benthic macrofauna community. This element includes establishing a sediment quality database, developing tools for interpreting chemistry data and measuring toxicity and benthic community responses, and integrating the tools into objectives that utilize a multiple line of evidence approach. The second element includes studies to investigate the relationship between sediment contamination and risks to humans and wildlife related to contaminant bioaccumulation (indirect effects). The third element involves developing guidance for use of tools (e.g., chemistry and biological analyses) for implementing SQOs in the context of existing State of California programs. The fourth study element will include a summary and assessment of sediment quality in California bays and estuaries that will incorporate the draft objectives developed in this project. Each element will be conducted in two phases. Phase I includes the development of tools for assessing direct effects in marine embayments. Phase II includes the development of tools for assessing direct effects in estuaries and the development of tools for assessing indirect effects in marine and estuarine habitats.

Tasks: Revise SQO development workplan. The 1991 SWRCB workplan for the development of sediment quality objectives will be revised and updated to reflect recent developments in the field of sediment quality assessment. A Scientific Steering Committee (SSC), composed of scientists from government agencies, universities, and research organizations, will be established to help guide the SQO development process. Meetings with a Sediment Quality Advisory Committee composed of stakeholder groups and an Agency Coordinating Committee composed of key state and federal agencies that use sediment quality guidelines will also be held in order to assist the state in identifying and resolving issues related to developing and implementing SQOs.

Establish statewide sediment quality database. Relevant and reliable sediment quality data from the entire state will be compiled and integrated into a relational database to support the SQO development and verification activities. This database will include all of the recent (i.e., last 10 years) available data on sediment contamination, toxicity, and benthic community impacts for marine and estuarine areas of California. The types of studies that will comprise the database will include the SWRCB Bay Protection and Toxic Cleanup studies, EMAP and other regional monitoring studies, NPDES monitoring, and site-specific dredging and TMDL studies. A database user’s manual will also be produced that describes the structure of the database and provides instructions for exporting information and adding new records.

Refine benthic community assessment tools. Statistically-based indices for assessing benthic community impacts have recently been developed for the Southern California Bight. These indices will be refined and analyses conducted to develop similar tools that are applicable to other regions of the state. Existing benthic community data from areas north of Point Conception will be analyzed to determine the adequacy of the data for index development in that portion of the state. Samples will be collected from selected locations in California bays and estuaries and analyzed for benthic community composition, contamination, and toxicity. These data will be used to develop improved tools to identify benthic community impacts from stress due to anthropogenic factors. This task will be conducted in parallel with additional benthic community assessment tool development projects at SCCWRP.

Assess relationships between contamination and effects. Analyses of the statewide database will be conducted to identify relationships among sediment contamination, habitat characteristics, and biological responses. The influence of differences in contamination patterns, sediment physical characteristics, and benthic community assemblages on response to contamination will be investigated. The efficiency, sensitivity, and specificity of selected sediment guidelines developed for other programs to predict biological effects in California will be evaluated.

Develop tools for assessing aquatic life impacts. The results of analyses conducted in prior tasks will be used to select methods for assessing three lines of evidence (i.e., benthic community health, toxicity, and sediment chemistry) that will be used to determine whether the narrative sediment quality objectives established by the State for direct effects have been met. Numeric thresholds that describe the level of impact present will be established for each line of evidence.

Develop tools for assessing bioaccumulation-related risks. The performance of various models to predict the accumulation of contaminants (i.e., the biota-sediment accumulation factor or BSAF) in the tissue of California sediment-associated fish and invertebrates will be investigated. Case studies in San Francisco Bay, Newport Bay, and the San Francisco Bay Delta will be conducted in an effort to evaluate methodologies for relating sediment contamination to exceedence of risk-based tissue thresholds in fish or shellfish consumed by humans or wildlife. The case studies will incorporate the application of bioaccumulation models and develop a data integration framework that identifies the process and factors necessary to assess whether sediment quality objectives for bioaccumulation-related impacts have been met.

Develop implementation program. Frameworks for integrating data from multiple lines of evidence and assessing impacts from direct and indirect effects will be developed. These frameworks will identify procedures to integrate data from multiple analyses that result in a categorical assessment of each station relative to the degree of impact present. Guidance manuals will be developed that describes appropriate procedures for the collection and analysis of sediment samples for chemical contamination, toxicity, benthic community condition, and bioaccumulation potential. Descriptions of methods to identify the cause of toxicity that are based on related SCCWRP research will be included (see Development of Methods to Characterize Sediment Toxicity in the Southern California Bight). The implementation program will also describe procedures for the use of SQOs in various activities, such as assessment of impaired habitats, cleanup of impacted areas, and monitoring. Different procedures may be developed for different types of applications.

Summarize sediment quality in bays and estuaries. A summary report will be prepared that describes the quality of sediments within California’s enclosed bays and estuaries. The report will summarize recent information on the extent of contamination and biological impacts, and also evaluate sediment quality using the SQOs developed under this program.

Project Status: This is the fifth year of a nine-year project. The development of work plans for various project elements, establishment of advisory and steering committees, compilation of sediment quality data and initiation of data analysis, and sampling to refine benthic indices for Phase I were completed during the first three years. The third and fourth years focused on data analyses, selection of tools for each line of evidence, and development of an assessment framework for implementing Phase I of the SQO program. The fifth year will focus on completing the implementation guidance and sediment quality assessment for Phase I, as well as planning for the Phase II activities.

Collaborators: This project is being conducted in collaboration with numerous regulated, regulatory and non-governmental organizations and is funded by the SWRCB.



C2     DEVELOPMENT OF METHODS TO CHARACTERIZE SEDIMENT TOXICITY IN THE SOUTHERN CALIFORNIA BIGHT
           Lead Investigator: S. Bay

Objectives: Sediment toxicity tests from regional monitoring in 1998 and 2003 showed significant toxicity to amphipods in both offshore, estuary, and Bay/Harbor areas. Identification of the cause(s) of toxicity is needed before the toxicity results from regional surveys, or other studies, can be used to guide management or regulatory responses, such as cleanup or TMDLs. Conventional toxicity tests and chemical analyses are rarely sufficient to identify the cause of toxicity because elevated concentrations of multiple contaminants are usually present in a toxic sample, along with changes in other important factors such as sediment organic carbon content or grain size. Focused toxicity identification evaluation (TIE) studies are needed to help determine the cause of toxicity.

An essential step in a TIE is characterization, in which experiments are conducted to determine the general classes of toxicants (e.g., metals, nonpolar organics) present. Standardized characterization methods are available for water samples (e.g., effluents) and usually consist of the addition of substances designed to remove or neutralize specific classes of toxicants. In contrast, toxicity characterization methods are rarely applied to sediments and no standard methodology has been developed. SCCWRP has been developing methods for southern California and initial results are promising. Still, insufficient information is available to allow the selection of those methods that are best able to discriminate among the complex mixture of chemicals often present in sediment samples.

The goal of this project is to further refine sediment toxicity characterization strategy for use in southern California. We will investigate chemical manipulations of bulk sediment and or interstitial water to alter contaminant bioavailability. In particular, we will focus on manipulations that will identify organic contaminants, such as pesticides, as potential toxicants of concern.

Tasks: Examination of bulk sediment and porewater TIE methods. Sediments from Newport Bay, San Diego Bay, and other locations will be tested using TIE methods to characterize the types of toxicants present in both bulk sediment and porewater. The results will be compared to determine whether each type of method identifies similar toxicant types (e.g., metals or nonpolar organics) as being present.

Development of methods to identify pesticide toxicity. The metabolic inhibitor piperonyl butoxide (PBO) has been successfully used to characterize organophosphate (op) pesticide toxicity in water samples, but the utility of this treatment for sediment is unknown. The efficacy of using PBO to eliminate sediment toxicity due to op pesticides will be examined using sediments spiked with diazinon or chlorpyrifos. Additional methods to identify toxicity due to pyrethroid pesticides will be investigated. Changes in toxicity will be measured using the amphipod Eohaustorius estuarius.

Evaluate sediment fractionation methods. Standard methods for extracting metal or organic contaminants from sediments are highly disruptive, nonspecific, and have limited compatibility with toxicity test procedures. Two methodologies for extracting toxicants from sediments for subsequent fractionation will be investigated. The first method will use the addition of resins designed to have an affinity for particular classes of contaminants to the sediment. The second method will use supercritical fluid extraction (SFE) for selectively extracting contaminants from sediments. Experiments will be conducted to determine whether these methods are compatible with sediment toxicity test procedures and whether variations in resin composition or SFE operating parameters are useful in characterizing or identifying sediment toxicants.

Investigate TIE accuracy and reproducibility. An interlaboratory comparison to document the comparability of sediment toxicity characterization procedures will be conducted. Toxic field and spiked sediments will be tested by multiple laboratories experienced in sediment TIE methods. Each lab will use similar TIE methods and the results will be compared to determine if the same types of toxicants are identified.

Project Status: This is the second year of a three-year study. Initial experiments to investigate methods to identify pesticide toxicity and extract sediments for fractionation were conducted in the first year. The second year will focus on refining the candidate extraction methods and evaluating the effectiveness of additional methods for identifying pesticide toxicity.

Collaborators: This study is being conducted in coordination with the U.S. EPA Environmental Research Laboratory in Narragansett (Rhode Island) and the UC Davis Environmental Toxicology Department.



C3     IN SITU MEASUREMENT OF TOXIC ORGANIC COMPOUNDS IN SEDIMENT POREWATER
           Lead Investigator: K. Maruya

Objectives: The State of California is rapidly progressing towards the development of sediment quality objectives (SQO) (). Measurements of the bioavailable fraction of pollutants such as polycyclic aromatic hydrocarbons (PAH), PCBs and chlorinated pesticides in contaminated estuarine sediments are key in the development and evaluation of SQOs.

Currently, measuring the dissolved, or bioavailable, fractions of trace organic contaminants is difficult and expensive. Frequently, existing methods lack the sensitivity needed to determine concentrations that can lead to aquatic life impairments. Passive methods that measure the dissolved phase fraction of sediment-associated pollutants offer clear advantages over traditional, costly and time-consuming ex situ techniques. However, passive methods to date have suffered from several challenges including predictive ability, reproducibility, and durability. SCCWRP has recently developed a new passive in situ sampler called solid phase microextraction (SPME) that are simple and inexpensive yet possess the sensitivity needed to address biological impacts. Previous deployments found trace organics in the water column overlying contaminated sediments.

The goal of this study is to develop and test an in situ sediment porewater sampler for hydrophobic organic pollutants based on SPME technology. Specifically, we will perform experiments to calibrate SPME fibers for a suite of environmentally relevant PAH, optimize the design and configuration of the SPME-based sampler, and determine the conditions under which the sampler can be deployed in laboratory assays as well under field conditions.

Tasks: Prototype sampler testing in sediment-water systems. A prototype sampler will be evaluated in sediment-water microcosms. Comparison of dissolved phase concentrations as measured by the SPME sampler with those determined by conventional techniques (i.e., porewater extractions) will be made over a range of sediment characteristics and pollutant concentrations. Predictions of SPME sampler performance using a theoretical model developed in Year 1 will be validated using sediment column experiments.

Comparing SPME bioavailability with invertebrate bioaccumulation. The prototype sampler will be co-exposed with live benthic organisms to native and radiolabeled HOCs amended into replicated bedded sediment-water microcosms using standard bioaccumulation test protocols. The effectiveness of the SPME sampler as a “biomimic” will be determined using the relationship between SPME-sorbed and tissue concentrations of model pollutants for different test species; spiked sediments with varying organic carbon content; and pristine and contaminated field-collected sediments.

Laboratory and data analysis. Loaded SPME fibers will be analyzed by thermal desorption GC/MS. Total pollutant mass sorbed by SPME fibers will be related to aqueous (porewater) concentrations and regressed against other measures of bioavailability (e.g., invertebrate body burdens). The expanded list of target analytes will include PAH, PCBs, DDTs, chlordanes and various other chlorinated hydrocarbon legacy contaminants.

Duration: This is the second year of a four-year study. Sampler prototypes were optimized in the first year. The second year will initiate prototype bioavailability testing.

Collaborators: This project is conducted in collaboration with the Chinese Academy of Sciences and is partially funded by the Cooperative Institute for Coastal and Estuarine Technology (CICEET).



C4    ESTIMATING POLLUTANT LOADINGS AND FLUXES IN IMPAIRED COOASTAL WATERWAYS
          Lead Investigator: K. Maruya

Objectives: Many of southern California’s creek mouths have relatively high levels of contamination, particularly those at the mouths of highly urbanized watersheds. As a result, urban creek mouths (i.e., Ballona Creek, Dominguez Channel, San Diego Creek, Chollas Creek, etc.) have been placed on the State of California’s list of impaired waterbodies. To restore these urban waterways, the State of California is developing total maximum daily loads (TMDLs) to minimize/reverse their impact on beneficial uses. To generate defensible TMDL targets, however, all major sources and transport pathways within a given waterbody must be characterized.

Because many classes of toxic compounds (e.g. metals, PAHs) are associated with erosional sediments and/or urban runoff, most effort to date has focused on quantifying pollutant loading during wet weather events. For persistent contaminants, including most legacy toxic organics (e.g., chlordanes, DDT, PCBs), present day loading due to wet weather events may be small compared with historic in situ sources such as bedded sediments. Thus, the contribution of dissolved and particulate-bound pollutant sources originating upstream relative to those originating from in situ sources are needed to apportion loading contributions. However, few if any of these measurements are made because they are logistically and technically difficult to accomplish. For example, the sensitivity of many methods is insufficient to achieve water column concentrations that are meaningful for estimating flux.

The objectives of this study are: 1) test and compare passive in situ water column samplers for measuring trace organics contaminants; 2) measure bedded sediment and water column concentrations of trace organic contaminants during low flow conditions; and 3) quantify source loading and flux through a coastal waterbody, including in-stream and bedded sediment sources, using a box model approach. New methods are becoming available and hold much promise (see In situ measurement of toxic organic compounds in sediment porewater), but comparisons among samplers are relatively untested. In this study, we will compare two of these new sampling techniques (solid phase microextraction vs. polyethylene extraction devices). Ultimately, this study will determine the relative importance of watershed vs. bedded sediment sources to the water column contributions of these trace organic contaminants.

Tasks: Compare performance of SPME and polyethylene devices (PEDs) for in situ measurements of organic pollutants. Using laboratory microcosms, calibration parameters for TMDL regulated organic pollutants (PAH, PCBs, DDTs, chlordanes, dieldrin) for both SPME fibers and PEDs will be catalogued. Sampler detection limits and precision for each device will be estimated under a range of environmental conditions (temperature, salinity, total suspended solids) expected in situ.

Site selection. Historical data on organic pollutants in the water column and sediments of Ballona Creek will be reviewed to determine sites for in situ SPME measurements and sediment grain size and metals analysis. A minimum of four sites in the Ballona Creek estuary will be selected for sampler deployment, with one site each located at the far upper (freshwater member) and lower (ocean member) reach of the estuary.

SPME deployment and sediment collection. During stable low flow conditions, SPME water column samplers will be deployed at multiple depths for up to 30 d. Discharge will be recorded over the deployment period so that dry-season loadings may be estimated.

Sediment and water column grain size/particle analysis. Bedded sediment grabs in depositional areas will be collected and analyzed for grain size, organic and trace metal pollutants in support of Year 2 partitioning and flux modeling. The particle size distribution of resuspended sediment will be characterized in situ (using a LISST 100X particle-size analyzer) near sediment collection stations.

Duration: This is the first year of a two-year study.

Collaborators: This project is conducted in collaboration with Loyola Marymount University (Dr. Rachel Adams) and is partially funded by the City of Los Angeles and the USC Sea Grant Program.



C5     EMERGING CONTAMINANTS OF CONCERN NI COASTAL WATERS, SEDIMENT, AND BIOTA
           Lead PI: K. Maruya

Objectives: Recent studies have suggested endocrine disruption in flatfish occurs near in the Los Angeles Margin and may be enhanced near large POTW outfalls (see Endocrine Disruption In Coastal Fish). Initial efforts to determine likely causative chemical agents, however, have been largely inconclusive. One reason is because the list of potential endocrine disrupting chemicals is large and increasing rapidly.

Although single chemical assays are easiest and thus most widely used, there are a myriad of environmental contaminants that are potential endocrine modulators. These include natural and synthetic hormones, industrial and commercial chemicals (e.g., nonylphenols, polybrominated diphenyl ethers), legacy compounds (e.g., DDT) and a new breed of consumer chemicals (pharmaceuticals and personal care products). Recent data suggests mixtures may be the most likely cause of any observed effects. Sampling and analysis of these multiple chemical classes in discharges has previously required substantial expertise, effort and cost. Little to no effort has been taken to measure these constituents in ambient receiving waters, particularly where they are matched with biological effects data.

The goal of this project is determine which classes of potential endocrine disrupting compounds are present in water, sediment, and fish from receiving water areas where biological effects of endocrine disruption are being observed. The initial focus of this study will be on the more hydrophobic contaminant classes (i.e., those that accumulate in sediment and/or tissues). Our recently proven in situ sampling technology based on solid phase microextraction (SPME) may prove sensitive for at least some of these more hydrophobic endocrine disrupting compounds. Ultimately, these data should prove useful in helping determine the chemical sources of these biological effects.

Tasks: Development of target (EDC) analyte-environmental matrix list. A master list of potential endocrine disrupting chemicals (EDCs) will be generated based on documented and/or suspected effects from the literature. The likelihood of occurrence in various environmental compartments (i.e., sediment, water column, biota) will be assessed using existing fate data and quantitative structure activity relationships.

Development of matrix-specific analytical methods for EDCs. Analytical protocols for EDC analyte-matrix combinations will be developed for GC- and LC-MS in collaboration with leading research analytical labs. SPME fibers will be calibrate