Agenda

U.S. EPA Workshop on Microbial Source Tracking

February 5, 2002

Marriott Hotel & Conference Center, Irvine, CA




7:30 - 8:30 Registration

Welcome
8:30 am - Gerard Stelma / Stephen Weisberg

Methods I (ribotyping, rep-PCR. PFGE, etc.)

8:45 - Lessons Learned and Questions Unanswered from 5 Years of Bacterial Source Tracking
        Valerie J. Harwood, Ph.D.
        Assistant Professor, Department of Biology
        University of South Florida

9:15 - Microbial Source Tracking: Principles and Practice
        Mansour Samadpour, Ph.D.
        Assistant Professor, Department of Environmental Health
        University of Washington

9:45 - Ribotyping Enterococci
        Peter G. Hartel, Ph.D.
        University of Georgia

Break

10:30 - Fecal Source Tracking with Bacteroides
        Katherine G. Field, Ph.D.
        Assistant Professor, Department of Microbiology
        Oregon State University

11:00 - Urbanization and Coastal Water Quality: What Can Molecular Fingerprinting Tell us?
        Patricia A. Holden, Ph.D.
        The Donald Bren School of Environmental Science & Management
        University of California, Santa Barbara

11:30 - Comparison of Environmental and Clinical Isolates of Escherichia coli using Various Genetic Fingerprinting Methods
        Cindy H. Nakatsu, Ph.D.
        Purdue University

12:00 - 1:30 Lunch Speaker:
Bacterial Endemism and Co-Speciation
        James T. Staley, Ph.D.
        Professor, Department of Microbiology
        University of Washington

1:30 - Source Tracking Fecal Bacteria in the Environment Using rep-PCR DNA Fingerprinting: Prospects and Problems
        LeeAnn K. Johnson
        Department of Soil, Water, and Climate
        University of Minnesota

Methods II (Antibiotic resistance profiles, biochemical profiles, immunological, etc.)

2:00 - Microbial Source Tracking Using Antibiotic Resistance Analysis
        Bruce A. Wiggins, Ph.D.
        Professor, Department of Biology
        James Madison University

2:30 - Animal Source Tracking: A Complement to Microbial Source Tracking
        R.D. Ellender, Ph.D.
        University of Southern Mississippi

3:00 - Carbon Source Profiles, Pulsed-field Gel Electrophoresis Patterns, and Antibiotic Resistance Analysis
        Charles Hagedorn, Ph.D.
        Professor, Department of Crop and Soil Environmental Sciences
        Virginia Polytechnic Institute and State University

Break

4:00 - Coliphage Tracking to Identify Sources of Fecal Contamination
        Mark D. Sobsey, Ph.D.
        Professor, Department of Environmental Sciences
        University of North Carolina at Chapel Hill

4:30 - Source Tracking of Fecal Waste Material in Environmental Waters using a Biomarker based on
Enterotoxin Genes in E. coli

        Betty Olson, Ph.D.
        Professor
        University of California, Irvine

5:00 - Detection of Enteroviruses Using PCR-Based Techniques for Source Identification and Assessment of
Microbiological Water Quality

        Rachel T. Noble, Ph.D.
        Assistant Professor, Institute of Marine Science
        University of North Carolina at Chapel Hill

5:30 - Adenovirus as an Index of Human Viral Contamination
        Sunny Jiang, Ph.D.
        Assistant Professor
        University of California, Irvine

CONCLUSION - Application of a methods to identify coliform pollution sources using multiple antibiotic resistance, selected molecular techniques and GIS spatial analysis
        Geoffrey Scott
        NOAA/NOS, Center for Coastal Environmental Health and Biomolecular Research

6:00 - Adjourn