David Wanless

Senior Research Technician

David Wanless is a Senior Research Technician/Lab Manager in the Microbiology Department at SCCWRP. He has developed and improved many source tracking assays and controls. David also has expertise in integrating automation into laboratory methods, and next-generation sequencing techniques.

He received his M. S. in marine biology from the University of Miami in 2012, and his B.S. in bacteriology from the University of Wisconsin in 1998. He joined SCCWRP in 2018.


M.S., marine biology, University of Miami, Miami FL, 2012.

B.S., bacteriology, University of Wisconsin, Madison, WI, 1998.

Professional Experience

Senior Research Technician/Lab Manager, Southern California Coastal Water research Project. Costa Mesa, CA. 2018-present

Draftsman, TL Sneed Consulting. La Canada, CA. 2017-2018

Research Technician, Cherokee Nations Technical services contractor for Walter Reed Army Institute of Research (WRAIR). Silver Spring, MD. 2013-2017

Research Associate 2, University of Miami - CIMAS/NOAA. Costa Mesa, CA. Miami, FL. 2005-2011

Consultant, Source Molecular. Miami, FL. 2008-2011

Laboratory Technician, Lark Technologies. Houston, TX. 2003-2005

Research Associate, Agensys. Santa Monica, CA. 2000-2002

Research Associate 2, Institute for Genomic Research. Rockville, MD. 1999-2000

Journal Articles

Joyner J, Wanless D, Sinigalliano CD, Lipp EK. Use of quantitative real-time PCR for direct detection of serratia marcescens in marine and other aquatic environments. Appl Environ Microbiol. 2014 Mar;80(5):1679-83. doi: 10.1128/AEM.02755-13. Epub 2013 Dec 27.

Schriewer A, Goodwin KD, Sinigalliano CD, Cox AM, Wanless D, Bartkowiak J, Ebentier DL, Hanley KT, Ervin J, Deering LA, Shanks OC, Peed LA, Meijer WG, Griffith JF, Santodomingo J, Jay JA, Holden PA, Wuertz S. Performance evaluation of canine-associated Bacteroidales assays in a multi-laboratory comparison study.Water Res. 2013 Jul 5

Sinigalliano CD, Ervin JS, Van De Werfhorst LC, Badgley BD, Ballesté E, Bartkowiak J, Boehm AB, Byappanahalli M, Goodwin KD, Gourmelon M, Griffith J, Holden PA, Jay J, Layton B, Lee C, Lee J, Meijer WG, Noble R, Raith M, Ryu H, Sadowsky MJ, Schriewer A, Wang D, Wanless D, Whitman R, Wuertz S, Santo Domingo JW. Multi-laboratory evaluations of the performance of Catellicoccus marimammalium PCR assays developed to target gull fecal sources. Water Res. 2013 Jul 5.

Shanks OC, Sivaganesan M, Peed L, Kelty CA, Blackwood AD, Greene MR, Noble RT, Bushon RN, Stelzer EA, Kinzelman J, Anan'eva T, Sinigalliano C, Wanless D, Griffith J, Cao Y, Weisberg S, Harwood VJ, Staley C, Oshima KH, Varma M, Haugland RA. Interlaboratory comparison of real-time PCR protocols for quantification of general fecal indicator bacteria. Environ Sci Technol. 2012 Jan 17;46(2):945-53

Abdelzaher AM, Wright ME, Ortega C, Hasan AR, Shibata T, Solo-Gabriele HM,Kish J, Withum K, He G, Elmir SM, Bonilla JA, Bonilla TD, Palmer CJ, Scott TM,Lukasik J, Harwood VJ, McQuaig S, Sinigalliano CD, Gidley ML, Wanless D, Plano LR, Garza AC, Zhu X, Stewart JR, Dickerson JW, Yampara-Iquise H, Carson C,Fleisher JM, Fleming LE. Daily measures of microbes and human health at a non-point source marine beach. J Water Health. 2011 Sep;9(3):443-57.

Shah AH, Abdelzaher AM, Phillips M, Hernandez R, Solo-Gabriele HM, Kish J,Scorzetti G, Fell JW, Diaz MR, Scott TM, Lukasik J, Harwood VJ, McQuaig S,Sinigalliano CD, Gidley ML, Wanless D, Ager A, Lui J, Stewart JR, Plano LR,Fleming LE. Indicator microbes correlate with pathogenic bacteria, yeasts and helminthes in sand at a subtropical recreational beach site. J Appl Microbiol.2011 Jun;110(6):1571-83.

Shibata T, Solo-Gabriele HM, Sinigalliano CD, Gidley ML, Plano LR, Fleisher JM, Wang JD, Elmir SM, He G, Wright ME, Abdelzaher AM, Ortega C, Wanless D, Garza AC, Kish J, Scott T, Hollenbeck J, Backer LC, Fleming LE. Evaluation of conventional and alternative monitoring methods for a recreational marine beach with nonpoint source of fecal contamination. Environ Sci Technol. 2010 Nov 1;44(21):8175-81.

Sinigalliano CD, Fleisher JM, Gidley ML, Solo-Gabriele HM, Shibata T, PlanoLR, Elmir SM, Wanless D, Bartkowiak J, Boiteau R, Withum K, Abdelzaher AM, He G, Ortega C, Zhu X, Wright ME, Kish J, Hollenbeck J, Scott T, Backer LC, Fleming LE.Traditional and molecular analyses for fecal indicator bacteria in non-point source subtropical recreational marine waters. Water Res. 2010 Jul;44(13):3763-72.

Baums IB, Goodwin KD, Kiesling T, Wanless D, Diaz MR, Fell JW. Luminexdetection of fecal indicators in river samples, marine recreational water, and beach sand. Mar Pollut Bull. 2007 May; 54(5):521-36.